2019


 

Papadopoulou AA, Müller SA, Mentrup T, Shmueli MD, Niemeyer J, Haug-Kröper M, von Blume J, Mayerhofer A, Feederle R, Schröder B, Lichtenthaler SF, Fluhrer R.

Signal Peptide Peptidase-Like 2c (SPPL2c) impairs vesicular transport and cleavage of SNARE proteins.

EMBO Rep. 2019 Feb 7. pii: e46451. doi: 10.15252/embr.201846451. [Epub ahead of print] PMID: 30733281

 

Niemeyer J, Mentrup T, Heidasch R, Müller SA, Biswas U, Meyer R, Papadopoulou AA, Dederer V, Haug-Kröper M, Adamski V, Lüllmann-Rauch R, Bergmann M, Mayerhofer A, Saftig P, Wennemuth G, Jessberger R, Fluhrer R, Lichtenthaler SF, Lemberg MK, Schröder B.

The intramembrane protease SPPL2c promotes male germ cell development by cleaving phospholamban.

EMBO Rep. 2019 Feb 7. pii: e46449. doi: 10.15252/embr.201846449. [Epub ahead of print]

 

Parhizkar S, Arzberger T, Brendel M, Kleinberger G, Deussing M, Focke C, Nuscher B, Xiong M, Ghasemigharagoz A, Katzmarski N, Krasemann S, Lichtenthaler SF, Müller SA, Colombo A, Monasor LS, Tahirovic S, Herms J, Willem M, Pettkus N, Butovsky O, Bartenstein P, Edbauer D, Rominger A, Ertürk A, Grathwohl SA, Neher JJ, Holtzman DM, Meyer-Luehmann M, Haass C.

Loss of TREM2 function increases amyloid seeding but reduces plaque-associated ApoE.

Nat Neurosci. 2019 Feb;22(2):191-204. doi: 10.1038/s41593-018-0296-9. Epub 2019 Jan 7. PMID: 30617257

 

Manuel Hitzenberger, Martin Zacharias

γ-Secretase Studied by Atomistic Molecular Dynamics Simulations: Global Dynamics, Enzyme Activation, Water Distribution and Lipid Binding
Front Chem. 2018; 6: 640. Published online 2019 Jan 4. doi: 10.3389/fchem.2018.00640 PMCID: PMC6328467


2018


Harald Steiner, Akio Fukumori, Shinji Tagami and Masayasu Okochi

Making the final cut: pathogenic amyloid-β peptide generation by γ-secretase

Cell Stress 2(11): 292-310. doi: 10.15698/cst2018.11.162

 

Meinl E, Thaler FS, Lichtenthaler SF.

Shedding of BAFF/APRIL Receptors Controls B Cells.

Trends Immunol. 2018 Sep;39(9):673-676. doi: 10.1016/j.it.2018.07.002. Epub 2018 Jul 30.

 

 

Lichtenthaler SF, Lemberg MK, Fluhrer R.

Proteolytic ectodomain shedding of membrane proteins in mammals-hardware, concepts, and recent developments.

EMBO J. 2018 Aug 1;37(15). pii: e99456. doi: 10.15252/embj.201899456. Epub 2018 Jul 5. Review.

 

 

Gotz A, Scharnagl C.

Dissecting conformational changes in APP's transmembrane domain linked to epsilon-efficiency in familial Alzheimer's disease.

PLoS One. 2018;13:e0200077.

 

 

Herber J, Njavro J, Feederle R, Schepers U, Muller U, Brase S, Muller S, Lichtenthaler SF.

Click chemistry-mediated biotinylation reveals a function for the protease BACE1 in modulating the neuronal surface glycoproteome.

Mol Cell Proteomics. 2018.

 

Kamp F, Scheidt HA, Winkler E, Basset G, Heinel H, Hutchison JM, LaPointe LM, Sanders CR, Steiner H, Huster D.

Bexarotene Binds to the Amyloid Precursor Protein Transmembrane Domain, Alters Its alpha-Helical Conformation, and Inhibits gamma-Secretase Nonselectively in Liposomes.

ACS Chem Neurosci. 2018.

 

 

Zellner A, Scharrer E, Arzberger T, Oka C, Domenga-Denier V, Joutel A, Lichtenthaler SF, Muller SA, Dichgans M, Haffner C.

CADASIL brain vessels show a HTRA1 loss-of-function profile.

Acta Neuropathol. 2018.

 

 

Högel P, Götz A, Kuhne F, Ebert M, Stelzer W, Rand KD, Scharnagl C, Langosch D.

Glycine Perturbs Local and Global Conformational Flexibility of a Transmembrane Helix.

Biochemistry. 2018 Feb 1.

 

Colombo A, Hsia HE, Wang M, Kuhn PH, Brill MS, Canevazzi P, Feederle R, Taveggia C, Misgeld Th, Lichtenthaler SF (2018).

Non-cell-autonomous function of DR6 in Schwann cell proliferation.

EMBO J. 2018

 

Fukumori, A., Trambauer, J., Feilen, L.P. & Steiner, H.

Photoaffinitätsmarkierung: Identifizierung von Substratbindestellen in der γ-Sekretase.

BIOspektrum, 2018-1, 34-36.

 

 

Brummer T, Pigoni M, Rossello A, Wang H, Noy PJ, Tomlinson MG, Blobel CP, Lichtenthaler SF.

The metalloprotease ADAM10 (a disintegrin and metalloprotease 10) undergoes rapid, postlysis autocatalytic degradation.

FASEB J. 2018:fj201700823RR.

 

 

Kronenberg-Versteeg, D., Eichmann, M., Russell, M.A., de Ru, A., Hehn, B., Yusuf, N., van Veelen, P.A., Richardson, S.J., Morgan, N.G., Lemberg, M.K., Peakman, M.

Molecular pathways for immune recognition of preproinsulin signal peptide in type 1 Diabetes.

Diabetes. 2018;67:687-96.

 


2017


Mentrup T, Loock AC, Fluhrer R, Schröder B.

Signal peptide peptidase and SPP-like proteases - Possible therapeutic targets?

Biochim Biophys Acta. 2017 Nov;1864(11 Pt B):2169-2182.

 

Schlepckow K, Kleinberger G, Fukumori A, Feederle R, Lichtenthaler SF, Steiner H, Haass C.

An Alzheimer-associated TREM2 variant occurs at the ADAM cleavage site and affects shedding and phagocytic function.

EMBO Mol Med. 2017 Oct;9(10):1356-1365.

 

Mentrup T, Fluhrer R, Schröder B.

Latest emerging functions of SPP/SPPL intramembrane proteases.

Eur J Cell Biol. 2017 Aug;96(5):372-382.

 

Jules F, Sauvageau E, Dumaresq-Doiron K, Mazzaferri J, Haug-Kröper M, Fluhrer R, Costantino S, Lefrancois S.

Kleinberger G, Brendel M, Mracsko E, Wefers B, Groeneweg L, Xiang X, Focke C, Deußing M, Suárez-Calvet M, Mazaheri F, Parhizkar S, Pettkus N, Wurst W, Feederle R, Bartenstein P, Mueggler T, Arzberger T, Knuesel I, Rominger A, Haass C.

The FTD-like syndrome causing TREM2 T66M mutation impairs microglia function, brain perfusion, and glucose metabolism.

EMBO J. 2017 Jul 3;36(13):1837-1853.

 

Julius A, Laur L, Schanzenbach C, Langosch D.

BLaTM 2.0, a Genetic Tool Revealing Preferred Antiparallel Interaction of Transmembrane Helix 4 of the Dual-Topology Protein EmrE.

J Mol Biol. 2017 Jun 2;429(11):1630-1637.

 

Scilabra SD, Yamamoto K, Pigoni M, Sakamoto K, Müller SA, Papadopoulou A, Lichtenthaler SF, Troeberg L, Nagase H, Kadomatsu K.

Langosch D, Steiner H.

Substrate processing in intramembrane proteolysis by γ-secretase - the role of protein dynamics.

Biol Chem. 2017 Apr 1;398(4):441-453.

 

Serdaroglu A, Müller SA, Schepers U, Bräse S, Weichert W, Lichtenthaler SF, Kuhn PH.

An optimised version of the secretome protein enrichment with click sugars (SPECS) method leads to enhanced coverage of the secretome.

Proteomics. 2017 Mar;17(5).

 

Baranger K, Bonnet AE, Girard SD, Paumier JM, García-González L, Elmanaa W, Bernard A, Charrat E, Stephan D, Bauer C, Moschke K, Lichtenthaler SF, Roman FS, Checler F, Khrestchatisky M, Rivera S.

Trambauer J, Fukumori A, Kretner B, Steiner H.


2016


Fleck, D., M. Voss, B. Brankatschk, C. Giudici, H. Hampel, B. Schwenk, D. Edbauer, A. Fukumori, H.

Steiner, E. Kremmer, M. Haug-Kroper, M. J. Rossner, R. Fluhrer, M. Willem & C. Haass

Proteolytic Processing of Neuregulin 1 Type III by Three Intramembrane-cleaving Proteases.

J Biol Chem, 2016; 291, 318-33

 

Müller SA, Scilabra SD, Lichtenthaler SF.

Front Mol Neurosci. 2016 Oct 13;9:96.

 

Pigoni M, Wanngren J, Kuhn PH, Munro KM, Gunnersen JM, Takeshima H, Feederle R, Voytyuk I, De Strooper B, Levasseur MD, Hrupka BJ, Müller SA, Lichtenthaler SF.

Seizure protein 6 and its homolog seizure 6-like protein are physiological substrates of BACE1 in neurons.

Mol Neurodegener. 2016 Oct 5;11(1):67.

 

Stelzer, W., Scharnagl, C., Leurs, U., Rand, K.D., Langosch, D.

"The Impact of the ‘Austrian’ Mutation of the Amyloid Precursor Protein Transmembrane Helix is Communicated to the Hinge Region"

ChemistrySelect, 2016; 1: 4403-4407.

Kretner, B., Trambauer, J., Fukumori, A., Mielke, J., Kuhn, P-H., Kremmer, E., Giese, A. , Lichtenthaler, S.F., Haass, C., Arzberger, T., Steiner, H. (2016):

"Generation and deposition of Aβ43 by the virtually inactive presenilin‐1 L435F mutant contradicts the presenilin loss‐of‐function hypothesis of Alzheimer's disease."

EMBO Mol Med. 2016;8:458-65.

 

Hüttl S, Helfrich F, Mentrup T, Held S, Fukumori A, Steiner H, Saftig P, Fluhrer R, Schröder B.

Biochem J. 2016 May 15;473(10):1405-22.

 

Kuhn PH, Colombo AV, Schusser B, Dreymueller D, Wetzel S, Schepers U, Herber J, Ludwig A, Kremmer E, Montag D, Müller U, Schweizer M, Saftig P, Bräse S, Lichtenthaler SF.

Systematic substrate identification indicates a central role for the metalloprotease ADAM10 in axon targeting and synapse function.

Elife. 2016 Jan 23;5. pii: e12748.

 


2015


Meissner C, Lorenz H, Hehn B, Lemberg MK.

Intramembrane protease PARL defines a negative regulator of PINK1- and PARK2/Parkin-dependent mitophagy.

Autophagy. 2015;11:1484-98.

 

 

 

Mentrup T, Hasler R, Fluhrer R, Saftig P, Schroder B.

A Cell-Based Assay Reveals Nuclear Translocation of Intracellular Domains Released by SPPL Proteases. Traffic. 2015;16:871-92.

 

 Kuhn PH, Voss M, Haug-Kroper M, Schroder B, Schepers U, Brase S, Haass C, Lichtenthaler SF, Fluhrer R. Secretome analysis identifies novel signal Peptide peptidase-like 3 (Sppl3) substrates and reveals a role of Sppl3 in multiple Golgi glycosylation pathways.

Mol Cell Proteomics. 2015;14:1584-98.

 

Winkler, E., Julius, A., Steiner, H., Langosch, D.

"Homodimerization Protects the Amyloid Precursor Protein C99 Fragment from Cleavage by γ-Secretase."
Biochemistry. 2015 Oct 13;54(40):6149-52

 

Langosch, D., Scharnagl, C.,  Steiner, H., Lemberg, M.K.

"Understanding intramembrane proteolysis: from protein dynamics to reaction kinetics."
Trends Biochem Sci. 2015 Jun;40(6):318-27