2019

Papadopoulou AA, Müller SA, Mentrup T, Shmueli MD, Niemeyer J, Haug-Kröper M, von Blume J, Mayerhofer A, Feederle R, Schröder B, Lichtenthaler SF, Fluhrer R.

Signal Peptide Peptidase-Like 2c (SPPL2c) impairs vesicular transport and cleavage of SNARE proteins.

EMBO Rep. 2019 Feb 7. pii: e46451. doi: 10.15252/embr.201846451. [Epub ahead of print] PMID: 30733281

Niemeyer J, Mentrup T, Heidasch R, Müller SA, Biswas U, Meyer R, Papadopoulou AA, Dederer V, Haug-Kröper M, Adamski V, Lüllmann-Rauch R, Bergmann M, Mayerhofer A, Saftig P, Wennemuth G, Jessberger R, Fluhrer R, Lichtenthaler SF, Lemberg MK, Schröder B.

The intramembrane protease SPPL2c promotes male germ cell development by cleaving phospholamban.

EMBO Rep. 2019 Feb 7. pii: e46449. doi: 10.15252/embr.201846449. [Epub ahead of print]

Parhizkar S, Arzberger T, Brendel M, Kleinberger G, Deussing M, Focke C, Nuscher B, Xiong M, Ghasemigharagoz A, Katzmarski N, Krasemann S, Lichtenthaler SF, Müller SA, Colombo A, Monasor LS, Tahirovic S, Herms J, Willem M, Pettkus N, Butovsky O, Bartenstein P, Edbauer D, Rominger A, Ertürk A, Grathwohl SA, Neher JJ, Holtzman DM, Meyer-Luehmann M, Haass C.

Loss of TREM2 function increases amyloid seeding but reduces plaque-associated ApoE.

Nat Neurosci. 2019 Feb;22(2):191-204. doi: 10.1038/s41593-018-0296-9. Epub 2019 Jan 7. PMID: 30617257

Manuel Hitzenberger, Martin Zacharias

γ-Secretase Studied by Atomistic Molecular Dynamics Simulations: Global Dynamics, Enzyme Activation, Water Distribution and Lipid Binding
Front Chem. 2018; 6: 640. Published online 2019 Jan 4. doi: 10.3389/fchem.2018.00640 PMCID: PMC6328467

2018

Harald Steiner, Akio Fukumori, Shinji Tagami and Masayasu Okochi

Making the final cut: pathogenic amyloid-β peptide generation by γ-secretase

Cell Stress 2(11): 292-310. doi: 10.15698/cst2018.11.162

Meinl E, Thaler FS, Lichtenthaler SF.

Shedding of BAFF/APRIL Receptors Controls B Cells.

Trends Immunol. 2018 Sep;39(9):673-676. doi: 10.1016/j.it.2018.07.002. Epub 2018 Jul 30.

Lichtenthaler SF, Lemberg MK, Fluhrer R.

Proteolytic ectodomain shedding of membrane proteins in mammals-hardware, concepts, and recent developments.

EMBO J. 2018 Aug 1;37(15). pii: e99456. doi: 10.15252/embj.201899456. Epub 2018 Jul 5. Review.

Gotz A, Scharnagl C.

Dissecting conformational changes in APP's transmembrane domain linked to epsilon-efficiency in familial Alzheimer's disease.

PLoS One. 2018;13:e0200077.

Herber J, Njavro J, Feederle R, Schepers U, Muller U, Brase S, Muller S, Lichtenthaler SF.

Click chemistry-mediated biotinylation reveals a function for the protease BACE1 in modulating the neuronal surface glycoproteome.

Mol Cell Proteomics. 2018.

Kamp F, Scheidt HA, Winkler E, Basset G, Heinel H, Hutchison JM, LaPointe LM, Sanders CR, Steiner H, Huster D.

Bexarotene Binds to the Amyloid Precursor Protein Transmembrane Domain, Alters Its alpha-Helical Conformation, and Inhibits gamma-Secretase Nonselectively in Liposomes.

ACS Chem Neurosci. 2018.

Zellner A, Scharrer E, Arzberger T, Oka C, Domenga-Denier V, Joutel A, Lichtenthaler SF, Muller SA, Dichgans M, Haffner C.

CADASIL brain vessels show a HTRA1 loss-of-function profile.

Acta Neuropathol. 2018.

Högel P, Götz A, Kuhne F, Ebert M, Stelzer W, Rand KD, Scharnagl C, Langosch D.

Glycine Perturbs Local and Global Conformational Flexibility of a Transmembrane Helix.

Biochemistry. 2018 Feb 1.

Colombo A, Hsia HE, Wang M, Kuhn PH, Brill MS, Canevazzi P, Feederle R, Taveggia C, Misgeld Th, Lichtenthaler SF (2018).

Non-cell-autonomous function of DR6 in Schwann cell proliferation.

EMBO J. 2018

Fukumori, A., Trambauer, J., Feilen, L.P. & Steiner, H.

Photoaffinitätsmarkierung: Identifizierung von Substratbindestellen in der γ-Sekretase.

BIOspektrum, 2018-1, 34-36.

Brummer T, Pigoni M, Rossello A, Wang H, Noy PJ, Tomlinson MG, Blobel CP, Lichtenthaler SF.

The metalloprotease ADAM10 (a disintegrin and metalloprotease 10) undergoes rapid, postlysis autocatalytic degradation.

FASEB J. 2018:fj201700823RR.

Kronenberg-Versteeg, D., Eichmann, M., Russell, M.A., de Ru, A., Hehn, B., Yusuf, N., van Veelen, P.A., Richardson, S.J., Morgan, N.G., Lemberg, M.K., Peakman, M.

Molecular pathways for immune recognition of preproinsulin signal peptide in type 1 Diabetes.

Diabetes. 2018;67:687-96.

2017

Mentrup T, Loock AC, Fluhrer R, Schröder B.

Signal peptide peptidase and SPP-like proteases - Possible therapeutic targets?

Biochim Biophys Acta. 2017 Nov;1864(11 Pt B):2169-2182.

Schlepckow K, Kleinberger G, Fukumori A, Feederle R, Lichtenthaler SF, Steiner H, Haass C.

An Alzheimer-associated TREM2 variant occurs at the ADAM cleavage site and affects shedding and phagocytic function.

EMBO Mol Med. 2017 Oct;9(10):1356-1365.

Mentrup T, Fluhrer R, Schröder B.

Latest emerging functions of SPP/SPPL intramembrane proteases.

Eur J Cell Biol. 2017 Aug;96(5):372-382.

Jules F, Sauvageau E, Dumaresq-Doiron K, Mazzaferri J, Haug-Kröper M, Fluhrer R, Costantino S, Lefrancois S.

CLN5 is cleaved by members of the SPP/SPPL family to produce a mature soluble protein.

Exp Cell Res. 2017 Aug 1;357(1):40-50.

Kleinberger G, Brendel M, Mracsko E, Wefers B, Groeneweg L, Xiang X, Focke C, Deußing M, Suárez-Calvet M, Mazaheri F, Parhizkar S, Pettkus N, Wurst W, Feederle R, Bartenstein P, Mueggler T, Arzberger T, Knuesel I, Rominger A, Haass C.

The FTD-like syndrome causing TREM2 T66M mutation impairs microglia function, brain perfusion, and glucose metabolism.

EMBO J. 2017 Jul 3;36(13):1837-1853.

Julius A, Laur L, Schanzenbach C, Langosch D.

BLaTM 2.0, a Genetic Tool Revealing Preferred Antiparallel Interaction of Transmembrane Helix 4 of the Dual-Topology Protein EmrE.

J Mol Biol. 2017 Jun 2;429(11):1630-1637.

Scilabra SD, Yamamoto K, Pigoni M, Sakamoto K, Müller SA, Papadopoulou A, Lichtenthaler SF, Troeberg L, Nagase H, Kadomatsu K.

Dissecting the interaction between tissue inhibitor of metalloproteinases-3 (TIMP-3) and low density lipoprotein receptor-related protein-1 (LRP-1): Development of a "TRAP" to increase levels of TIMP-3 in the tissue.

Matrix Biol. 2017 May;59:69-79.

Langosch D, Steiner H.

Substrate processing in intramembrane proteolysis by γ-secretase - the role of protein dynamics.

Biol Chem. 2017 Apr 1;398(4):441-453.

Schanzenbach C, Schmidt FC, Breckner P, Teese MG, Langosch D.

Identifying ionic interactions within a membrane using BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions.

Sci Rep. 2017 Mar 7;7:43476.

Serdaroglu A, Müller SA, Schepers U, Bräse S, Weichert W, Lichtenthaler SF, Kuhn PH.

An optimised version of the secretome protein enrichment with click sugars (SPECS) method leads to enhanced coverage of the secretome.

Proteomics. 2017 Mar;17(5).

Baranger K, Bonnet AE, Girard SD, Paumier JM, García-González L, Elmanaa W, Bernard A, Charrat E, Stephan D, Bauer C, Moschke K, Lichtenthaler SF, Roman FS, Checler F, Khrestchatisky M, Rivera S.

MT5-MMP Promotes Alzheimer's Pathogenesis in the Frontal Cortex of 5xFAD Mice and APP Trafficking in vitro.

Front Mol Neurosci. 2017 Jan 10;9:163.

Trambauer J, Fukumori A, Kretner B, Steiner H.

Analyzing Amyloid-β Peptide Modulation Profiles and Binding Sites of γ-Secretase Modulators.

Methods Enzymol. 2017;584:157-183.

2016

Fleck, D., M. Voss, B. Brankatschk, C. Giudici, H. Hampel, B. Schwenk, D. Edbauer, A. Fukumori, H.

Steiner, E. Kremmer, M. Haug-Kroper, M. J. Rossner, R. Fluhrer, M. Willem & C. Haass

Proteolytic Processing of Neuregulin 1 Type III by Three Intramembrane-cleaving Proteases.

J Biol Chem, 2016; 291, 318-33

Müller SA, Scilabra SD, Lichtenthaler SF.

Proteomic Substrate Identification for Membrane Proteases in the Brain.

Front Mol Neurosci. 2016 Oct 13;9:96.

Pigoni M, Wanngren J, Kuhn PH, Munro KM, Gunnersen JM, Takeshima H, Feederle R, Voytyuk I, De Strooper B, Levasseur MD, Hrupka BJ, Müller SA, Lichtenthaler SF.

Seizure protein 6 and its homolog seizure 6-like protein are physiological substrates of BACE1 in neurons.

Mol Neurodegener. 2016 Oct 5;11(1):67.

Stelzer, W., Scharnagl, C., Leurs, U., Rand, K.D., Langosch, D.

"The Impact of the ‘Austrian’ Mutation of the Amyloid Precursor Protein Transmembrane Helix is Communicated to the Hinge Region"

ChemistrySelect, 2016; 1: 4403-4407.

Kretner, B., Trambauer, J., Fukumori, A., Mielke, J., Kuhn, P-H., Kremmer, E., Giese, A. , Lichtenthaler, S.F., Haass, C., Arzberger, T., Steiner, H. (2016):

"Generation and deposition of Aβ43 by the virtually inactive presenilin‐1 L435F mutant contradicts the presenilin loss‐of‐function hypothesis of Alzheimer's disease."

EMBO Mol Med. 2016;8:458-65.

Fukumori, A. & Steiner, H.

"Substrate recruitment of γ-secretase and mechanism of clinical presenilin mutations revealed by photoaffinity mapping."

EMBO J. 2016;35:1628-43.

Hüttl S, Helfrich F, Mentrup T, Held S, Fukumori A, Steiner H, Saftig P, Fluhrer R, Schröder B.

Substrate determinants of signal peptide peptidase-like 2a (SPPL2a)-mediated intramembrane proteolysis of the invariant chain CD74.

Biochem J. 2016 May 15;473(10):1405-22.

Kuhn PH, Colombo AV, Schusser B, Dreymueller D, Wetzel S, Schepers U, Herber J, Ludwig A, Kremmer E, Montag D, Müller U, Schweizer M, Saftig P, Bräse S, Lichtenthaler SF.

Systematic substrate identification indicates a central role for the metalloprotease ADAM10 in axon targeting and synapse function.

Elife. 2016 Jan 23;5. pii: e12748.