Project P1: Substrate Recognition and Cleavage by the Mitochondrial Rhomboid Protease PARL
Rhomboids are universally conserved intramembrane serine proteases that impact on a variety of important cellular processes.
While analysis of bacterial rhomboids has provided first insights of how cognate transmembrane domains are selected for cleavage, for the more distant mitochondrial rhomboid protease PARL still only very little is known.
Central aim of this project within the FOR2290 is to decipher how PARL selects its substrates. This is of particular relevance since PARL has an active site topology opposite to classical rhomboid proteases in bacteria and the eukaryotic secretory pathway, indicating that it has evolved a unique substrate selection mechanism.
Yücel SS, Lemberg MK.
J Mol Biol. 2020 Aug 21;432(18):5063-5078. doi: 10.1016/j.jmb.2020.05.014. Epub 2020 May 26. PMID: 32464132 Review.
Yücel SS, Stelzer W, Lorenzoni A, Wozny M, Langosch D, Lemberg MK.
Cell Rep. 2019 Mar 12;26(11):3087-3099.e11. doi: 10.1016/j.celrep.2019.02.057. PMID:30865896
Lichtenthaler SF, Lemberg MK, Fluhrer R.
EMBO J. 2018 Aug 1;37(15). pii: e99456. doi: 10.15252/embj.201899456. Epub 2018 Jul 5. Review.
Kronenberg-Versteeg D, Eichmann M, Russell MA, de Ru A, Hehn B, Yusuf N, van Veelen PA, Richardson SJ, Morgan NG, Lemberg MK, Peakman M.
"Understanding intramembrane proteolysis: from protein dynamics to reaction kinetics."
Langosch, D., Scharnagl, C., Steiner, H., Lemberg, M.K. (2015)
Trends Biochem Sci. 2015 Jun;40(6):318-27