Project P8: Investigation of Molecular Dynamics of Substrate Transmembrane α-Helices by Solution and Solid-State NMR Spectroscopy
In this project, we investigate the structure and dynamics of transmembrane (TM) proteases’ substrates using a combination of solution and solid-state NMR spectroscopy. As the exact mechanism by which transmembrane proteases recognize their substrates is still not clear, we investigate if the molecular dynamics of a TM helix may determine its suitability as a substrate for intramembrane proteolysis.
To this end, we will apply numerous NMR techniques in solution and in membrane reconstituted systems. In close collaborations with the cell biology and computer simulation groups within the consortium this approach will shed more light on the mechanism by which transmembrane proteases recognize/bind and process their substrates.
Götz A, Mylonas N, Högel P, Silber M, Heinel H, Menig S, Vogel A, Feyrer H, Huster D, Luy B, Langosch D, Scharnagl C, Muhle-Goll C, Kamp F, Steiner H.
Biophys J. 2019 Jun 4;116(11):2103-2120. doi: 10.1016/j.bpj.2019.04.030. Epub 2019 May 3. PMID: 31130234
Kamp F, Scheidt HA, Winkler E, Basset G, Heinel H, Hutchison JM, LaPointe LM, Sanders CR, Steiner H, Huster D.
ACS Chem Neurosci. 2018.